Slot blot hybridization of membrane-immobilized, single-stranded human DNA with the higher primate-specific alphoid probe D17Z1 is routinely used in forensic science to estimate the amount of DNA in biological samples.Typically, a chemiluminescent signal captured on film records the hybridization, and the quantity of the signal is Nail Treatments related to the amount of immobilized DNA.Digital imaging using a cooled CCD camera offers an alternate non-film-based method for image acquisition with comparable sensitivity of detection, a greater dynamic range, enhanced capability of data interpretation, and often faster results than film.
In addition, the data support the premise that more accurate and precise #4 MEDIUM BROWN human DNA quantification should be obtained by not assuming a linear response of signal to known standards.Instead, quantity should be estimated using a second-order standard curve (R2 = 0.999).
Finally, a CCD camera imaging system offers versatility for image capture of different signal sources and analysis of samples on a variety of support media.